rptec growth kit Search Results


htert immortalized rptec growth kit  (ATCC)
95
ATCC htert immortalized rptec growth kit
Analysis of human proximal tubular albumin reuptake function. Albumin uptake by human proximal tubular epithelial cells after 15 min incubation at 37 °C with 50 µg mL-1 of FITC-albumin (green) added to the channel under static conditions ( A ) and fluid shear stress conditions ( B ). Mean Fluorescence Intensity (MFI) of <t>RPTEC/TERT1</t> cells 24 h after static conditions versus fluid shear stress conditions showing a significant increase in FITC-Albumin fluorescence signal ( C ) (***p ≤ 0.001). Transcriptome profiling provides insight to differentially expressed genes corresponding to endocytosis process.
Htert Immortalized Rptec Growth Kit, supplied by ATCC, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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htert immortalized rptec growth kit - by Bioz Stars, 2026-02
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Analysis of human proximal tubular albumin reuptake function. Albumin uptake by human proximal tubular epithelial cells after 15 min incubation at 37 °C with 50 µg mL-1 of FITC-albumin (green) added to the channel under static conditions ( A ) and fluid shear stress conditions ( B ). Mean Fluorescence Intensity (MFI) of RPTEC/TERT1 cells 24 h after static conditions versus fluid shear stress conditions showing a significant increase in FITC-Albumin fluorescence signal ( C ) (***p ≤ 0.001). Transcriptome profiling provides insight to differentially expressed genes corresponding to endocytosis process.

Journal: Scientific Reports

Article Title: Three dimensional modeling of biologically relevant fluid shear stress in human renal tubule cells mimics in vivo transcriptional profiles

doi: 10.1038/s41598-021-93570-5

Figure Lengend Snippet: Analysis of human proximal tubular albumin reuptake function. Albumin uptake by human proximal tubular epithelial cells after 15 min incubation at 37 °C with 50 µg mL-1 of FITC-albumin (green) added to the channel under static conditions ( A ) and fluid shear stress conditions ( B ). Mean Fluorescence Intensity (MFI) of RPTEC/TERT1 cells 24 h after static conditions versus fluid shear stress conditions showing a significant increase in FITC-Albumin fluorescence signal ( C ) (***p ≤ 0.001). Transcriptome profiling provides insight to differentially expressed genes corresponding to endocytosis process.

Article Snippet: Human immortalized hRPTECs (RPTEC/TERT1, ATCC CRL-4031) and were cultured and maintained in hTERT Immortalized RPTEC Growth Kit (ATCC ACS-4007), supplemented with Geneticin (Gibco, 10131035), in phenol red free DMEM/F-12 medium (Gibco, 11039021) according to the vendor's instructions.

Techniques: Incubation, Shear, Fluorescence

Fluorescent transporter substrate Calcein-AM and CMFDA amasses in RPTEC/TERT1 cells under static conditions and dissipates with the application of FSS. Figure ( B ) and ( D ) sample fluorescence images demonstrate decreased in Calcein-AM and CMFDA accumulation in cells after 24 h of FSS treatment prior to staining compared to static channels ( A and C ) (Bar = 100 µm). ( E ) Quantitative analysis of Mean Fluorescence Intensity (MFI) of Calcein-AM showed a significant decrease after cells were treated with 24 h of FSS versus static environment (yellow filled bars). Indicating the increased efflux activity after being kept under fluidic conditions (Bar = 100 µm; n = 4, ***p ≤ 0.001).

Journal: Scientific Reports

Article Title: Three dimensional modeling of biologically relevant fluid shear stress in human renal tubule cells mimics in vivo transcriptional profiles

doi: 10.1038/s41598-021-93570-5

Figure Lengend Snippet: Fluorescent transporter substrate Calcein-AM and CMFDA amasses in RPTEC/TERT1 cells under static conditions and dissipates with the application of FSS. Figure ( B ) and ( D ) sample fluorescence images demonstrate decreased in Calcein-AM and CMFDA accumulation in cells after 24 h of FSS treatment prior to staining compared to static channels ( A and C ) (Bar = 100 µm). ( E ) Quantitative analysis of Mean Fluorescence Intensity (MFI) of Calcein-AM showed a significant decrease after cells were treated with 24 h of FSS versus static environment (yellow filled bars). Indicating the increased efflux activity after being kept under fluidic conditions (Bar = 100 µm; n = 4, ***p ≤ 0.001).

Article Snippet: Human immortalized hRPTECs (RPTEC/TERT1, ATCC CRL-4031) and were cultured and maintained in hTERT Immortalized RPTEC Growth Kit (ATCC ACS-4007), supplemented with Geneticin (Gibco, 10131035), in phenol red free DMEM/F-12 medium (Gibco, 11039021) according to the vendor's instructions.

Techniques: Fluorescence, Staining, Activity Assay